Production of CCHF Virus-Like Particle by a Baculovirus-Insect Cell Expression System
- Author:
Zhaorui ZHOU
;
Manli WANG
;
Fei DENG
;
Tianxian LI
;
Zhihong HU
;
Huafin WANG
- Publication Type:Journal Article
- Keywords:
Crimean-Congo hemorrhagic fever virus(CCHFV);
Virus-Like Particle(VLP);
Nucleocapsid(N)protein
- From:
Virologica Sinica
2011;26(5):338-346
- CountryChina
- Language:Chinese
-
Abstract:
Crimean-Congo Haemorrhagic Fever Virus(CCHFV)is a tick-born virus of the Nairovirus genus within the Bunyaviridae family,which is widespread and causes,high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research,the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope,Virus-Like Particles (VLPs)with various size and morphology were observed in cytoplasmic vesicles in the infected cells.Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation,which was confirmed by Western blot analysis and immuno-electron microscopy(IEM).
