Intravascular biocompatibility of poly (3-hydroxybutyrate- co-3-hydroxyhexanoate)
10.3969/j.issn.1673-8225.2011.38.009
- VernacularTitle:3-羟基丁酸与3-羟基己酸共聚酯的血管内生物相容性
- Author:
Song WU
;
Yinglong LIU
;
Yue TANG
;
Qiang WANG
;
Feng WAN
;
Xianghua QU
;
Guoqiang CHEN
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(38):7066-7070
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: The degradable poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) has superior mechanical property and biocompatibility.OBJECTIVE: To elucidate the intravascular biocompatibility of PHBHHx in vivo.METHODS: We developed hybrid materials based on decellularized xenogenic vascular scaffolds that were coated with PHBHHx and implanted it into the abdominal aorta of New Zealand rabbits. The decellularized xenogenic pulmonary artery patch without PHBHHx coating served as the control. The implanted patches were determined for the histology, immunofluorescence staining, scanning electron microscopy and calcium contents at 1, 4 and 12 weeks after the surgery.RESULTS AND CONCLUSION: Hybrid patches exhibited smooth lumen surface without thrombus, the intimal hyperplasia was mild and recellularization was complete; immunofluorescence staining showed that the endothelial cells in the neointima were positive for CD31, with continuous single-layer arrangement, interstitial cells were positive for smooth muscle actin; the calcium content in hybrid patches was obviously lower than that in uncoated patches. PHBHHx shows a remarkable intravascular biocompatibility in vivo and is believed as an ideal candidate for lumen coating of cardiovascular tissue engineering.
