Chitosan hydrogel microsphere-templated microcapsules carrying heparin via layer-by-layer self-assembly
10.3969/j.issn.1673-8225.2011.38.015
- VernacularTitle:壳聚糖水凝胶微球模板的载肝素层层自组装微胶囊
- Author:
Yingfeng WU
;
Haili GUO
;
Juan LI
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(38):7095-7099
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Electrostatic layer-by-layer (LbL) self-assembly is a simple, effective and novel approach to carrying macromolecules into microcapsule carriers.OBJECTIVE: To prepare chitosan (CS) microcapsules to control the releasing of heparin (Hep) by LbL.METHODS: CS microspheres were fabricated by a sodium sulfate-based precipitation process and then used as positively charged templates for polyelectrolyte multilayer coatings to carry heparin by the LbL self-assembly technique. The polycation was CS and the polyanion was Hep. The microcapsule was formed via coating {CS/Hep}3 on CS hydrogel template. The microcapsules composed of {CS/ Hep}3 on CS hydrogel templates were confirmed by fluorescence inverted microscopy, confocal laser scanning microscopy and laser particle size analysis. The assembly of polyelectrolyte layers on CS hydrogel microspheres was monitored by Zeta Potential Analyzer.RESULTS AND CONCLUSION: The resulting microcapsules were about 1 μm in average diameter, and allowed spontaneous loading of heparin through electrostatic interaction, with the encapsulation efficiency and carrying capacity of 83.8% and 3.05%, respectively.