Expressions of CD34,Delta-like-1 protein in hepatic regeneration tissue of rats after partial hepatectomy
10.3969/j.issn.1673-8225.2011.31.029
- VernacularTitle:大部分肝切除模型大鼠肝再生组织中CD34蛋白及Delta-like-1蛋白的表达**☆
- Author:
Zhiyun YANG
;
Yixin HOU
;
Xianbo WANG
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(31):5822-5826
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: When the liver affected severe damage or excalation, liver stem cells rapidly proliferated and differentiated into mature liver cells to repair liver tissue. However, liver regeneration is a complicated multiple signal molecular regulatory process and related genes and proteins that participated in regulation remain unclear. OBJECTIVE: To investigate the expressions of CD34 and Delta-like-1 protein in rat liver regenerated tissues after partial hepatectomy. METHODS: Rat hepatic regeneration models were established after hepatectomy. Expressions of ATPase, proliferating cell nuclear antigen (PCNA), CD34 and Delta-like-1 protein were detected by hematoxylin-eosin staining, enzyme-histochemistry and immunohistochemistry method at various time points. RESULTS AND CONCLUSION: At the early stage of liver regeneration, the expression of ATPase was down-regulated, and the expressions of CD34 and Delta-like-1 protein were up-regulated. At the middle stage, the expression of ATPase was increased to the normal level, and the expressions of CD34 and Delta-like-1 protein were down-regulated. The expression of PCNA protein was continuously up-regulated. These suggest that the expressions of ATPase, CD34 and Delta-like-1 protein were different at different stages of hepatic regeneration, which means that different signal molecules were cooperated during the hepatic regeneration, and Notch-Delta signal pathway takes part in the pathogenesy.
