Effects of different concentrations of recombinant human erythropoietin on proliferation of neural stem cells cultured in vitro
10.3969/j.issn.1673-8225.2011.23.004
- VernacularTitle:不同浓度重组人促红细胞生成素对神经干细胞体外培养增殖的影响
- Author:
Zhengmin XUE
;
Meng HU
;
Changhai ZHANG
;
Xiancheng ZHANG
;
Xiaopeng ZHOU
- Publication Type:Journal Article
- From:
Chinese Journal of Tissue Engineering Research
2011;15(23):4194-4198
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND: Recombinant human erythropoietin (rhEPO) is a glycoprotein. Recent studies have demonstrated that rhEPO regulates many functional activities of neural cells. OBJECTIVE: To investigate the effects of different concentrations of rhEPO on proliferation of neural stem cells (NSCs) cultured in vitro. METHODS: Newborn Sprague-Dawley rat NSCs were harvested and cultured with serum-free culture medium containing different concentrations (5, 50, 500 U/mL) of rhEPO and 20 μg/L basic fibroblast growth factors (5, 50, and 500 U/mL rhEPO groups) and serum-free culture medium only containing 20 μg/L basic fibroblast growth factors (control group). After 7 days of culture, the cloning efficiency of NSCs was calculated. After 10 days of culture, neuron specific enolase (NSE)-and glial fibrillary acidic protein (GFAP)-immunoreactive cells were quantified. RESULTS AND CONCLUSION: In the rhEPO groups, cells proliferated rapidly, and the number of NSC microspheres was greater, in particular in the 50 U/mL rhEPO group, compared with the control group. NSCs grew faster in the 50 U/mL rhEPO group than in the control group. The number of NSE-and GFAP-immunoreactive cells was greater in the 50 U/mL rhEPO group than in the control group (P<0.01). These findings suggest that rhEPO promotes the in vitro culture and proliferation of NSCs, in particular 50 U/mL rhEPO.