Characterization and Diagnostic Use of a Monoclonal Antibody for VP28 Envelope Protein of White Spot Syndrome Virus
10.1007/s12250-011-3202-0
- Author:
Chonglin HOU
;
Yu CAO
;
Ronghui XIE
;
Yizhen WANG
;
Huahua DU
- Publication Type:Journal Article
- Keywords:
White spot syndrome virus (WSSV);
Recombinant VP28;
Monoclonal antibody (MAb)
- From:
Virologica Sinica
2011;26(4):260-266
- CountryChina
- Language:Chinese
-
Abstract:
The gene encoding the VP28 envelope protein of White spot syndrome virus (WSSV)was cloned into expression vector pET-30a and transformed into the Escherichia coli strain BL21.After induction,the recombinant VP28 (rVP28) protein was purified and then used to immunize Balb/c mice for monoclonal antibody (MAb) production.It was observed by immuno-electron microscopy the MAbs specific to rVP28 could recognize native VP28 target epitopes of WSSV and dot-blot analysis was used to detect natural WSSV infection.Competitive PCR showed that the viral level was approximately 104 copies/mg tissue in the dilution of gill homogenate of WSSV-infected crayfish at the detection limit of dot-blot assay.Our results suggest that dot-blot analysis with anti-rVP28 MAb could rapidly and sensitively detect WSSV at the early stages of WSSV infection.
