Expression and localization of Kuzbanian-DN mRNA in trans-gene NOD mouse pancreas cells
- VernacularTitle:转基因 NOD 小鼠胰腺 Kuzbanian-DNmRNA 的表达定位
- Author:
Xuemin WANG
- Publication Type:Journal Article
- Keywords:
In situ hybridization histochemistry
- From:
Chinese Journal of Diabetes
2008;16(10):586-588
- CountryChina
- Language:Chinese
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Abstract:
Objective Kuzbanian (KUZ) is the functional homologue of the vertebrate ADAM10. ADAMs are a protein family of cell surface-anchored membrane, whose members have extensive biological activities. This paper was to explore KUZ-DN (the dominant negative construct of KUZ) mRNA expression and localization in the trans-gene NOD mouse pancreas tissue and to provide molecular basis for the mechanism of the gene intervened stem cell differentiation to treat diabetes. Methods Pancreas was frozen and paraffin sections of pancreas in eight trans-gene NOD mice were made. Non-radioactivity in situ hybridization experiment was conducted on the tissue sections by the cRNA probes of KUZ-DN (360 bp) sense and antisense with DIG labeled. Results There was the strong positive KUZ-DN mRNA signal report of in situ hybridization in the trans-gene NOD mouse pancreas cells. Sense chain probes and blank comparison sections were negative. Conclusion The positive expression and localization of KUZ-DN mRNA in the trans-gene NOD mouse pancreas cells provides a vital data for the gene therapy research project of the KUZ-DN intervened Notch/Delta signaling pathway by the stem cell differentiation to treat diabetes