The effbct of hjgh-fat diet on IRS-1 and IRS-2 expression of fatry liver in rat
- VernacularTitle:高脂饮食对大鼠脂肪肝胰岛素受体底物1、2表达的影响
- Author:
Shiqing CHEN
;
Hua LI
;
Qi LIU
- Publication Type:Journal Article
- Keywords:
High-fat diet;
Insulin resistance;
Insulin receptor substrate
- From:
Chinese Journal of Diabetes
2008;16(7):409-412
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of high-fat diet on IRS-1 and IRS-2 expressions of fatty liver in rat. Methods After the rats were fed a high-fat diet (n=7) and a standard chow(n=7) for 8 weeks, insulin sensitivity,lipid metabolism, TNF-α and hepatic mRNA and protein expressions of IRS-1 and IRS-2 were measured. Results Compared with the normal control, the high-fat diet group displayed the elevated levels of insulin resistance,FFA and TNF-α.Hepatic IRS-1 mRNA expression and IRS-1 protein content were reduced by 28% and 32%(P<0.01),respectively. Similarly, hepatic IRS-2 mRNA and IRS-2 protein content were also reduced by 30% and 27% (P<0.01), respectively. Conclusions High-fat diet results in a significant decrease in expressions of mRNA and protein of hepatic IRS-1 and IRS-2 in rats, which might be one of the molecular mechanisms of insulin resistance in liver steatosis induced by high-fat diet
