Type 2 diabetes in MKR transgenic mice cansed by skeletal muscle-specific double deficiency of IGF-1 and insulin receptors
- VernacularTitle:骨骼肌特异性胰岛素样生长因子1及胰岛素双受体功能缺失所致小鼠2型糖尿病
- Author:
Rong YU
;
Xihua CHENG
;
Wei HU
;
Kaichun WEI
;
Yubing GUO
;
Junli LIU
- Publication Type:Journal Article
- Keywords:
Diabetes,type 2;
Transgenic technology;
Insulin-like growth factor 1 receptor;
Insulin receptor
- From:
Chinese Journal of Diabetes
2008;16(7):438-440
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the circulating glucose and body weight in the transgenic MKR mouse model who expressed dominant-negative IGF-1 receptor and insulin receptor in skeletal muscle leading to systemic insulin resistance and diabetes. Methods MKR mice were genotyped by PCR analysis of tail DNA.And in these mice we examined the circulating glucose and body weight once a week from 1 to 16 weeks of age, and the circulating insulin and glucose tolerance at age of two-month-old by using C57 mice as controls. Results The descendents of MKR mice kept hereditary feature. And these mice had hyperglycaemia from 3 weeks of age,and an increasing body weight slowly(P<0.01).Twenty-fold significant hyperinsulinemia was observed in MKR mice,and they were glucose intolerant in 2-month-old male and female (P<0.01).Conclusions The MKR mouse is an excellent model of type 2 diabetes
