Measurement of transforming growth factor beta 1 mRNA expression in renal cortex of diabetic rats using real-time reverse transcriptase-polymerase chain reaction with SYBR Green I
- VernacularTitle:应用SYBR Green I荧光定量RT-PCR测定糖尿病大鼠肾皮质TGF-β1表达水平
- Author:
Yingjian CHEN
;
Xiaowen TU
;
Chengjin HU
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus
- From:
Chinese Journal of Diabetes
2006;14(2):148-149
- CountryChina
- Language:Chinese
-
Abstract:
Expression levels of TGF-β1 mRNA in renal cortex of control group, diabetic group and taurine group measured by real-time quantitative RT-PCR were (7.0±0.8)×10 -3, (64.4±8.0)×10 -3 and (16.7±2.0)×10 -3, respectively. The corresponding results with end-point RT-PCR method were 0.28±0.12,0.58±0.16 and 0.43±0.10, respectively. Compared with end-point RT-PCR method, real-time quantitative with SYBR Green I was easier, faster, sensitive and specific.
