Effect of reduced glutathione pretreatment on inflammatory factors and oxygen free radical during one-lung ventilation
10.3760/cma.j.issn.1673-4904.2011.33.010
- VernacularTitle:还原型谷胱甘肽预处理对单肺通气患者围手术期炎性因子和自由基的影响
- Author:
Yuanqiang LI
;
Zhigang WANG
;
Benjing GONG
- Publication Type:Journal Article
- Keywords:
Glutathione;
Inflammation;
One-lung ventilation
- From:
Chinese Journal of Postgraduates of Medicine
2011;34(33):25-27
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of reduced ghtathione (GSH) pretreatment on inflammatory factors and oxygen free radical in patients during one-lung ventilation (OLV).MethodsThirty patients with lung cancer undergoing lung resection were divided into GSH group(15 cases) and control group ( 15 cases) by random digits table.In GSH group,GSH 30 mg/kg in normal saline 100 ml was infused after induction of anesthesia before OLV,while in control group equal volume of normal saline was infused instead of GSH.Blood samples were collected before induction of anesthesia (T0) and at 30 min(T1),60 min (T2) of OLV and 60 min of two-lung ventilation (T3) and at 2 h after operation(T4) for determination of serum tumor necrosis factor(TNF)-α,interleukin(IL)-8 and malonaldehyde(MDA) concentrations and superoxide dismutase(SOD) activity.ResultsThe serum MDA concentrations at T2-T4 were higher than those at T0 in two groups (P＜ 0.05) [GSH group:(3.5 ± 0.6),(3.8 ± 0.8),(4.7 ± 1.1 ),(6.1 ± 1.2),(6.4 ± 0.9) mol/L at T0-T4;control group:(3.7 ±0.5),(4.1 ±0.6),(5.9 ± 1.2),(7.4 ± 1.0),(7.8 ± 1.1) mol/L at T0-T4],but GSH group was lower than control group(P＜ 0.05 ).The serum SOD activity at T2-T4 was lower than that at T0 in control group (P＜ 0.05),and lower than GSH group(P＜ 0.05).The serum TNF-α,IL-8 concentrations at T1-T4 were higher those that at T0 in two groups(P ＜ 0.05 ),the serum TNF- α concentrations at T2-T4 and IL-8 concentrations at T1-T4 in GSH group were lower than those in control group (P ＜0.05).Conclusion Pretreatment with GSH 30 mg/kg can decrease inflammatory response and lipid peroxidation during OLV.
