Ultrasound imaging of acute renal allograft rejection with microbubbles targeted to intercellular adhesion molecule-1
10.3760/cma.j.issn.1004-4477.2011.12.021
- VernacularTitle:细胞间黏附分子-1靶向微泡超声造影成像评价肾移植后急性排异反应
- Author:
Lijing JI
;
Baoping WANG
;
Lihong LUO
;
Fenglin WU
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Kidney transplantation;
Graft rejection;
Intercellular adhesion molecule-1
- From:
Chinese Journal of Ultrasonography
2011;20(12):1070-1073
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo assess the feasibility of evaluation of renal allograft acute rejection in rat withcontrast-enhancedultrasound( CEUS )andtargetedmicrobubbles.MethodsPhospholipid microbubbles targeted to intercellular adhesion molecule -1 (ICAM-1)(MBI) and control microbubbles (MB) were created by conjugating monoclonal antibody against ICAM-1 or isotype control antibody to the lipid capsule via “avidin-biotin” bridging.Ten SD rats with acute renal allograft rejection were injected intravenous of MBI and MB in random order with a 30-min interval.After 3 min of intravenous injection of microbubbles,targeted CEUS imaging was performed in all rats.And then the video intensity (VI) was determined.ResultsIn MBI group,a significant ultrasonic enhancement was observed,but it was not very obvious in MB group.Increment in VI value of transplant kidney in MBI group was great and it amounted to (27.0 ± 7.4)U,however,increment in VI value of in MB group was minor and it was merely (10.2 ± 2.4) U,Difference was evident in transplant kidney between of the two groups (P ＜ 0.05).Conclusions Molecular imaging of ICAM-1 with targeted CEUS can evaluate renal allograft acute rejection effectively.
