Value of contrast-enhanced ultrasonography in evaluating the protective effect of ischemic postconditioning after rabbit's testis compltely torsion and detorsion
10.3760/cma.j.issn.1004-4477.2011.12.023
- VernacularTitle:超声造影在评价缺血后处理对兔睾丸完全扭转复位后保护效应中的应用
- Author:
Yu WANG
;
Ensheng XUE
;
Yu ZHANG
;
Qianqian CHEN
;
Rongxi LIANG
;
Shun CHEN
;
Liwu LIN
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Spermatic cord torsion;
Ischemic preconditioning;
Reperfusion injury
- From:
Chinese Journal of Ultrasonography
2011;20(12):1074-1078
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo assess the protective effect of ischemic postconditioning in different ways after rabbit's testis completely torsion and detorsion and to assess the value of contrast-enhanced ultrasonography(CEUS) in it.MethodsForty male white rabbits were divided into 5 groups randomly:a control group,directly perfusion group (R),ischemic postconditioning groups (IP1,IP2 and IP3),with 8 in each.The acute testicular hemodynamic disorder models were established in R,IP1,IP2,IP3 groups by completely blocking the left testicular blood flow for 4 hours,then group R was directly subjected to perfusion,group IP1 was subjected to 15 s release and 15 s occlusion (15 s/15 s),IP2 was subjected to 30 s/ 30 s,IP3 was subjected to 45 s/45 s,each group was taken 3 cycles.All the groups underwent CEUS before operation and after 3 days,examined for the contents of malonaldehyde(MDA),superoxide dismutase(SOD) and histology,and observed for the pathobgieal changes of the testicular tissue.ResultsThe CEUS parameters of bilateral testes showed no significantly difference in all group before operation( P ＞0.05),3 days after reperfusion.The half of declining time of intensity was extended significantly in all operation groups comparing with control group (P ＜ 0.05),but the difference of peak and base intensity were increased in group IP1 and IP2,comparing with control group( P ＜0.05).The contents of MDA and SOD were remarkable differences in group IP1,IP2 comparing with group R and IP3 ( P ＜ 0.05).Johnsen' s score,apoptosis index showed significantly differences in group IP1,IP2 comparing with group R and IP3 (P ＜ 0.05).ConclusionsMicrocirculation of the complete torsion testicular was significantly improved by ischemic postconditioning after reperfusion,and the effects of ischemic postconditioning could be evaluated by CEUS.
