Study on the changes of the protein conformation of gestational diabetes mellitus (GDM) placenta tissue by using Raman spectroanalysis
- VernacularTitle:利用拉曼光谱分析妊娠糖尿病患者胎盘组织蛋白质构象的变化
- Author:
Ying CHANG
;
Xiumin NIU
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus,gestational;
Placenta;
Raman spectrum;
Protein conformation
- From:
Chinese Journal of Diabetes
2008;16(5):294-296
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protein conformational change of placenta tissue by using Raman spectrum.Methods By using Raman spectroanalysis,we detected the placenta protein conformational change of GDM and control groups.Results(1)In the placenta of GDM,the absorption bands of tryptophan and phenylalanine were increased obviously.(2)In the placenta of GDM,the secondary structure of protein was composed of α-helix,random coil and β-sheet.Conclusions In the placenta of GDM,the orderly conformations of main chains in protein are decreased.Side chains of amino acids,especially tryptophan and phenyl-alanine,are changed greatly.The structure variation of protein may be correlated to the diabetic complications.
