Effect of Anti - idiotype Antibody on Anti - DNA Antibody Production by Hybridoma Cells.
- Author:
Jung Koo YOUN
;
Hyung Il KIM
;
Joo Deuk KIM
;
Sun PARK
;
Young Tai KIM
;
Milli Na LEE
- Publication Type:In Vitro ; Original Article
- Keywords:
Anti-idiotype antibody;
Isotype;
Ab production
- MeSH:
Antibody Formation*;
B-Lymphocytes;
Cell Proliferation;
DNA*;
Genes, Immunoglobulin;
Hybridomas*;
Immunoglobulin G;
Immunoglobulin M;
Immunoglobulins;
Lymphocytes
- From:Korean Journal of Immunology
1997;19(4):513-524
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Anti-idiotype antibody (anti-id Ab) which recognizes idiotope in the variable region of immunoglobulin (Ig) can regulate Ab production by B cells in vivo and in vitro. Although it has been reported that anti-id Ab can suppress IgM production by lymphocytes or hybridoma cells without suppression of cell proliferation, the regulatory mechanism of anti-id Ab is not completely understood. We studied the effects of anti-id Ab on the production of IgG class anti-DNA Ab by hybridoma cells, on the proliferation of cells, and on the transcription levels of Ig genes. In contrast to suppressive effect of anti-id Ab on the production of IgM previously reported by others, stimulatory effects of anti-id Ab on the production of IgG by hybridoma cells as well as the proliferation of these .cells were observed. However, little effect of anti-id Ab on the transcription levels of Ig genes was observed. These results suggest that anti-id Ab can increase Ab production by stimulation of cell proliferation. Furthermore, these results suggest that the effect of anti-id Ab on the production of Ab may be determined by the difference in class of Ab produced by hybridoma cells following the treatment with anti-id Ab.
