The effect of sphingosine kinase 1 on biological characteristics of human hepatocellular carcinoma cell line BEL-FU
10.3760/cma.j.issn.1007-8118.2011.12.014
- VernacularTitle:鞘氨醇激酶1对肝癌耐药细胞生物学特性的影响
- Author:
Haiying CHEN
;
Yanjing GAO
;
Huiya LIU
;
Dalei JIANG
;
Yong YUAN
;
Mei LI
;
Xin MA
- Publication Type:Journal Article
- Keywords:
Sphingosine kinase 1;
HCC;
Multidrug resistance-associated protein
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(12):1001-1004
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the roles of sphingosine kinasel (SPK1) in apoptosis,invasiveness and multidrug resistance of human hepatocellular carcinoma cell line BEL-FU.Methods BEL-FU cells were infected with adenovirus carrying SPK1wT gene and SPK1siRNA (Ad-H1-SPK1) gene.Their effects on biological characteristics of BEL-FU cells were evaluated by MTT,cellular SPK enzyme activity assay,Transwell Migration Technology and Western-blot,respectively.Results AdSPK1wT significantly increased SPK activity but SPK1siRNA(Ad-H1-SPK1) decreased SPK activity.Over expression of SPK1 suppressed the apoptosis induced by DMS(Dimethyl sphingosine,DMS) and enhanced migration of BEL-FU cells.The cells infected with SPK1 siRNA( Ad-H1-SPK1)significantly increased the apoptosis induced by DMS and inhibited the migration of human hepatocellular carcinoma cells.The expression of multidrug resistance-related protein (MRP1) of cells infected with SPK1siRNA (Ad-H1-SPK1) was suppressed significantly compared with the control group,while the expression of MRP1 infected with Ad- SPK1wT was enhanced.Conclusion SPK1 activity is closely associated with apoptosis、migration and multidrug resistance of human hepatocellular carcinoma cells,therefore,it may serve as a new target for HCC treatment.
