Evaluation of angiogenesis after stem cell transplantation using color-encoded parametric quantification with targeted microbubbles in rabbits with myocardial infarction
10.3760/cma.j.issn.1004-4477.2011.11.021
- VernacularTitle:彩色编码参数量化技术结合靶向微泡造影剂评价干细胞移植后微血管新生
- Author:
Guiqiu CAO
;
Yuming MU
;
Yanhong LI
;
Qianqian ZENG
;
Chunmei WANG
;
Tao JIANG
- Publication Type:Journal Article
- Keywords:
Ultrasonography;
Microbubbles;
Myocardial infarction;
Stem cell transplantation;
Neovascularization
- From:Chinese Journal of Ultrasonography
2011;20(11):983-987
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the application of parametric quantification (PQ) with targeted microbubbles on angiogenesis after stem cell transplantation in rabbits with myocardial infarction.Methods A CD34-targeted microbubble was prepared.Acute myocardial infarction (AMI) model were established by ligating coronary artery in 80 New Zealand white rabbits.Rabbits in common microbubble group (T + C group) and CD34-targeted microbubble group (T + T group) were subjected to myocardial contrast echocardiography(MCE),then compare myocardial perfusion parameters (A,β and A × β values) pre- and 3d post- ligation and 4 weeks after stem cell transplantation.Microvessel density (MVD) were determine by CD34 antibody immunohistochemistry.Results Compared to before stem cell transplantation,the perfusion scores ( P ＜0.05) and A,β and A× β values( P ＜0.01) of T+ C group and T+ T group were increased than before transplantation and control group.MVDs after stem cell transplantation in experimental groups were higher than those of control group ( P ＜0.01),especially the T + T group.A × β value in the T + C group was correlated with MVD ( r =0.658,P ＜ 0.05) ; β and A × β value in the T + T group were correlated with MVD ( r =0.620,0.859,P ＜0.05).A × β value ( X ) and MVD ( Y ) were included to establish the following regression equation:Y =- 130.986 + 34.505 X ( R 2 =0.556,P ＜ 0.05).Conclusions PQ and CD34-targeted microbubble probably provides some value in invasive evaluation of angiogenesis after stem cell transplantation.
