Curcumin affects expression of HGF and TGF-β1 of lung tissue in pulmonary fibrosis
10.3760/cma.j.issn.1008-1372.2011.11.008
- VernacularTitle:姜黄素对肺纤维化大鼠肺组织TGF-β1与HGF表达的影响
- Author:
Xia XIAO
;
Zhenhua HE
- Publication Type:Journal Article
- Keywords:
Curcumin/PD;
Pulmonary fibrosis/ME;
Lung/DE/ME;
Transforming growth factor betal/DE/ME;
Hepatocyte growth factor/DE/ME
- From:
Journal of Chinese Physician
2011;13(11):1468-1472
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the effect of Curcumin on TGF-β1 and HGF in pulmonary fibrosis and the effect of different concentrations of Curcumin on the expression of HGF and TGF-β1 in pulmonary fibrosis.Methods90 health male SD rats were randomly divided into six groups as follows:the control group ( group A),model group ( group B),prednisolone-treated group ( group C),and groups of low ( D group),middle( group E) and high( group F) dose curcumin (50,100,and 200 mg/kg,respectively).Animal model of pulmonary fibrosis was produced in SD rats by the endotracheal bleomycin,samples were obtained on days 7,14,and 28.The expression of HGF and TGF-β1 were measured by RT-PCR and Westem blot.ResultsThe light density values (0.693 ±0.028) and gray value (0.96 ±0.10)of TGF-β1 on day 28 of group A was the lowest,while the light density values ( 1.586 ±0.020) and gray value ( 1.77 ±0.15 ) of group B was the highest,the light density values (0.881 ± 0.032) and gray value ( 1.19 ±+ 0.12 )of group F was lower than group B.But the light density value and gray value of HGF showed reverse,the light density values and gray value of HGF on day 28 of group F was the highest,there was no statistical difference between group B and group D ( P ＞ 0.05).ConclusionsThe protective effect of Cucumin against pulmonary fibrosis may be through stimulating the activity of HGF and inhibiting the activity of TGFβ1 in a dose-dependent manner.
