Combined therapeutic effects of Huaiqihuang and Montelukast sodium on airway inflammation and expression of IL-17 in asthmatic rat
10.3760/cma.j.issn.1008-1372.2011.11.013
- VernacularTitle:槐杞黄联合孟鲁司特钠干预对哮喘大鼠气道炎症以及IL-17表达的影响
- Author:
Tao WANG
;
Qingnan HE
;
Hongmei LI
- Publication Type:Journal Article
- Keywords:
FRUCTUS LYCII/PD;
POLYGONATUM KINGIANUM/PD;
Leukotriene antagonists/PD;
Quinolines/PD;
Asthma/PA/ME/ZJ;
Respiratory tract diseases/PA;
Interleukin- 17/ME
- From:
Journal of Chinese Physician
2011;13(11):1484-1488
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the mechanism of Montelukast combined with Huang Qi Huai in the treatment of asthma.MethodsForty male Sprague-Dawley (SD) rats were chosen and randomly divided into five groups:control group (Control),model group (Model),Montelukast group (MK),Huang Qi Huai group (H),Montelukast + Huang Qi Huai group (MK + H),each group has 8 rats.The other four groups except the control group were built to chronic rat asthma model.The treatment groups were administered intragastrically with Montelukast,Huang Qi Huai and Montelukast + Huang Qi Huai respectively.All animals were sacrificod; plasma and bronchoalveolar lavage fluid (BALF) and superior lobe of right lung tissues were collected.Superior lobes of right lung tissues were used to measure the expression of IL-17 in lung tissue by immunohistochemistry.The airway inflammation was analyzed by histochemistry staining with H.E.Total cells score and differential score in BALF were counted.The levels of IL-17 in the plasma and BALF were measured by Enzyme-linked immunosorbent assay (ELISA).ResultsCompared with the control group,the degree of inflammatory cell around the airway in the model group were significantly higher (P ＜ 0.05).Compared with group model,the degree of inflammatory cell around the airway in the Group MK,group H and group MK + H were significantly ameliorated ( P ＜0.05).Compared with Group MK,the degree of inflammatory cell around the airway in group MK + H was significantly ameliorated ( P ＜O.05),the expression of IL-17 in lung tissues was significantly lower ( P ＜0.05),the numbers of total cells and the concentration of IL-17 in plasma and BALF were decreased too ( P ＜0.05).Correlation analysis showed that the level of IL-17 in the plasma and BALF was positively correlated with the level of IL-17 in the lung tissue( P ＜ 0.05 ).ConclusionsCombined Huang Qi Huai adjuvant Montelukast treatment of asthmatic rat further reduced the concentration of IL-17 in the plasma and BALF,reduced the expression of IL-17 in lung tissue,improved the airway inflammation.Down regulation the expression of IL-17 was probably one of the mechanisms of anti-inflammatory.
