The effects of TGF-β1 on the apoptosis of nerve cells after spinal cord injury in rats
10.3760/cma.j.issn.0253-2352.2011.12.009
- VernacularTitle:转化生长因子-β1对脊髓损伤后神经细胞凋亡的影响
- Author:
Qingpeng LIU
;
Meng YAO
;
Songruo LIN
;
Xianguang WANG
;
Changwei ZHOU
;
Chongyi SUN
- Publication Type:Journal Article
- Keywords:
Spinal cord injuries;
Transforming growth factor-batal;
Apoptosis
- From:
Chinese Journal of Orthopaedics
2011;31(12):1344-1351
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effects of exogenous transforming growth factor β1 on the apoptosis of nerve cells and its functions following spinal cord injury in rats.MethodsNinety-six male Wistar rats were randomly divided into 4 groups:group A(control group),group B(spinal cord injured group),group C (spinal cord injuried +TGF-β1 treated group),group D (spinal cord injuried +anti-TGF-β1 treated group).The rat model of spinal cord injury was found by the Allen's method.In group C and D,drugs were injected into subarachnoid cavity continuously by minipump.The changes of spinal cord were observed by HE staining.Nerve cells apoptosis was detected by transferase-medi-ated dUTP nick end labeling (TNUEL) method and the expression of cell apoptosis factor Fas by immunohistochemistry staining.Functional recovery of hind limbs was measured by Basso-Beattie-Bresnahan locomotor open field behavioral rating test.ResultsThe expression of TGF-β1and Fas increased following spinal cord injury.The amount of TGF-β1and Fas reached the peak 7days and 1day after injury.The apoptosis of neuron had increased and peaked 8 hours after injury.The apoptosis of neuroglia cells had increased and peaked 7 dayss after injury.The slight changes was found in spinal cord in group C.Both of the number of apoptosis of nerve cells and expression of the apoptosis factor (Fas) decreased significantly; compare with group B and D.The BBB score was higher in group C than that in group B and D.ConclusionTGF-β1 can improve the functional recovery of spinal cord by inhibiting the nerve cell apoptosis after the spinal cord injury.
