Liquid chromatography-mass spectrometry for measurement of serum 25 ( OH ) D level

VernacularTitle:血清25(OH)D测定的液相色谱质谱法
Author: Jianlie ZHOU ; Yue CHEN ; Chen YU ; Shuijun LI
Publication Type:Journal Article
Keywords: Vitamin D; liquid chromatography-mass spectrometry; 25 (OH) D2; 25 (OH) D3
From: Chinese Journal of Clinical Nutrition 2011;19(5):339-342
CountryChina
Language:Chinese
Abstract: Many diseases are associated with vitamin D deficiency.Serum 25 (OH)D levels can serve as objective indicators of the nutritional status of vitamin D.Liquid chromatography-mass spectrometry (LC-MS) method may be applied for routine measurement of 25 ( OH ) D levels in clinical practice.This article reviewed the significance of detecting 25 (OH) D2 and 25 (OH) D3,compared the advantages and disadvantages of different 25 (OH) D measurement methods,and introduced the application of LC-MS method for routine detection of serum 25 (OH) D2 and 25 (OH) D3 levels following the standard by National Institute of Standards and Technology of America.