Study on radiosensitivity by targeting HIF-1α in human lung cancer and growth of the transplanted tumors
10.3760/cma.j.issn.0254-5098.2011.06.005
- VernacularTitle:HIF-1α基因沉默对人肺癌放射敏感性及移植瘤生长的影响
- Author:
Jieqing LI
;
Xianrang SONG
;
Wei LIU
;
Jinming YU
;
Dianjun HOU
;
Ya MA
;
Li FENG
;
Jianwei QIAO
- Publication Type:Journal Article
- Keywords:
Hypoxia;
HIF-1 α;
Lung cancer;
Radiosensitivity
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(6):640-643
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the radiosensitivity by targeting HIF-lα in human lung cancer and the effects on tumor growth in nude mice.Methods Radiosensitivity of A549 and A549/HIF-1α ( － ) cells were tested by clonogenic forming assay.A549/HIF-1 α( － ) cells and A549 cells were injected into the male BALB/C nude mice.Tumor growth was observed.The expression of HIF-1α and microvessel density were detected by immunohistochemistry method.Results SERs of HIF-1α gene silencing were 1.03 in normoxia and 1.65 in hypoxia.The sizes of tumor xenografts derived from A549/HIF-1α( － ) cells were significantly reduced compared to those of the xenografts derived from A549 cells.HIF-1 α protein staining result showed a dramatic decrease in tumors from A549/HIF-1α ( － ) mice.The microvessel densities (MVD) were 19.83 ± 4.09 in A549 group and 11.61 ±3.04 in A549/HIF-lα (-)group(F=15.57,P ＜0.05 ).Conclusions Hypoxia-induced radio-resistance in lung cancer A549 cells could be reversed by silencing the HIF-1α.It also retards the growth of tumor xenografts,decreases HIF-1α expression and reduces the vascularity.
