Dose-effect relationship of mRNA level of human sensitive mitochondrial genes in lymphoblastoid cells induced by 60Co γ-rays
10.3760/cma.j.issn.0254-5098.2011.06.002
- VernacularTitle:60Co γ射线诱导人淋巴细胞线粒体基因表达效应的研究
- Author:
Hui WANG
;
Jiangbin FENG
;
Xue LU
;
Yuwen LI
;
Hua ZHAO
;
Li FAN
;
Deqing CHEN
;
Qingjie LIU
- Publication Type:Journal Article
- Keywords:
60 Co γ-rays;
Human lymphoblastoid cells;
Sensitive mitochondrial genes;
Gene expression;
Dose-effect relationship
- From:
Chinese Journal of Radiological Medicine and Protection
2011;31(6):627-630
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the dose-effect relationship of mRNA level of sensitive mitochondrial genes in human lymphoblastoid cells induced by ionizing radiation.Methods Seven human sensitive genes,including ND3,Cyt b,COX Ⅰ,COX Ⅱ,COX Ⅲ,ATPase6 and ATPase8 were chosen.Changes of mRNA level of these genes were detected by RT-PCR and Real-Time PCR at 24 h after irradiation in human lymphoblastoid cells,which were exposed to 0 - 15 Gy of 60 Co γ-rays.Results The expression of these 7 genes at mRNA level was up-regulated 24 h after irradiation in human lymphoblastoid cells.The level of gene expression of COX Ⅰ,which belongs to complex Ⅳ of mitochondrial respiratory chain,was most obvious,and the peak occurred after irradiation of 8 Gy,which was 13 times of the control group.A good dose-effect relationship was showed for COX Ⅲ gene expression at dose range of 3 -10 Gy as well as 3 - 15 Gy for other 3 genes including ND3,ATPase6 and ATPase8.Conclusions Gene expression levels of COX Ⅲ,ND3,ATPase6 and ATPase8 24h post-irradiation at certain irradiation dose range could be used for radiation damage biomarkers.
