Expression of GDNF and ChAT in the hippocampus of STZ-induced diabetic rats and relation between GD-NF and cognitive function
10.3760/cma.j.issn.1008-6706.2011.24.006
- VernacularTitle:糖尿病大鼠海马区胶质细胞源性神经营养因子和胆碱乙酰转氨酶mRNA表达及其与认知功能的关系
- Author:
Guoping YIN
;
Li CHEN
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Cognitive dysfunction;
Glial cell line-derived neurotrophic factor;
Choline acetyltransferase;
Reverse transcription,polymerase chain reaction
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(24):3325-3327
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the relationship between GDNF and ChAT Mrna and their potential role in the diabetic cognitive dysfunction by observing the change of the expression of GDNF and ChAT mRNA in brain under different blood glucose levels in STZ induced diabetic rats.MethodsForty male Wistar rats were randomly divided into diabetic group without blood glucose control( DM1 group),diabetic rats treated with insulin group( DM2 group),and normal control group( NC group).Blood glucose and body weight were detected every 4 weeks.Twelve weeks later,the hippocampus were submitted for cryostat sections which were subjected to the reverse transcription-polymerase chain reaction(RT-PCR) for GDNF and ChAT mRNA.ResultsThe expression of GDNF mRNA and ChAT mRNA in the hippocampus have negative correlation with HbAlc level( r =-0.962,-0.974,all P ＜ 0.001 );The GDNF expression had positive correlation with the ChAT mRNA expression( r =0.974,P ＜ 0.001 ).ConclusionLongterm chronic hyperglycation caused the “downregulation” of the GDNF expression in the hippocampal,which could cause the central cholinergic dysfunction symbled with the decrease of ChAT mRNA expression and the decline of learning and cognition ability.
