The diagnostic value of fluorescence quantitative polymerase chain reaction for the detection of respiratory tract infection of children with EB virus
10.3760/cma.j.issn.1008-6706.2011.23.018
- VernacularTitle:荧光定量聚合酶链反应检测对呼吸道感染患儿EB病毒的诊断价值
- Author:
Wang LEI
- Publication Type:Journal Article
- Keywords:
Herpesvirus;
Respiratory tract infections;
Polymerase chain reaction
- From:
Chinese Journal of Primary Medicine and Pharmacy
2011;18(23):3202-3203
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the fluorescence quantitative polymerase chain reaction and teat its diagnostic value of DNA(EBV-DNA)of EB virus in respiratory tract infection in children.Methods To adopt fluorescent quantitative PCR technology(FQ-PCR)to examine EBV-DNA in the aspirate specimen from 127 cases of children.Results 68 cases of EBV-DNA positive(53.5％),including 1year of age:12;3 years of age:23;5 years of age:18;7 years of age:10;9 years of age:3 and 13 years of age:2.The number of peripheral white blood cells ＞ 10.0× 109/L:21 cases;the number of white blood cells ＜ 10.0 × 109/L:8 cases;the number of mononuclear cells ＞ 10％:9 cases;abnormal lymphocyte ＞ 10％:10 cases;thrombocytopenia:4 cases;abnormal liver fumction:5 cases; myocardial enzyme abnormalities:8 cases.Conclusion The fluorescent quantitative PCR technology to test EB-DBA of respiratory tract infection in children had hight diagnostic value.
