The expression and correlation of programmed cell death 5 and tumor necrosis factor-alpha in rheumatoid arthritis patients
10.3760/cma.j.issn.1007-7480.2011.11.006
- VernacularTitle:类风湿关节炎患者程序化死亡基因5与肿瘤坏死因子-α的表达及其相关性分析
- Author:
Shaolong ZHANG
;
Zhenpeng GUAN
;
Junfeng WANG
;
Huan PAN
;
Zheng PEI
;
Ning WANG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Osteoarthritis;
Serum;
Synovial fluid;
Apoptosis;
Tumor necrosis factor-alpha
- From:
Chinese Journal of Rheumatology
2011;15(11):746-748
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo detect the expression level of programmed cell death (PDCD) 5 and tumor necrosis factor(TNF)-α in serum and joint fluid from rheumatoid arthritis (RA) and osteoarthritis (OA)patients,and analyze the correlation between PDCD5 and TNF-α in order to study the role of PDCD5 in the pathogenesis of RA.MethodsFiftypatients(including 26 RA,24 OA) between December 2009 and August 2010 were selected to this study.ELISA was used to detect the concentration of PDCD5 and TNF-α in the serum and joint fluid.Two-independent sample t-test and Pearson's correlation analysis were used for statistics.ResultsIn both serum and joint fluid,the concentration of PDCD5 from RA patients [(37±33) vs (37±26) pg/ml ] was significantly higher than that of OA patients [ ( 13± 14) vs ( 11 ±7 ) pg/ml ] (P＜0.05).The concentration of TNF-α in the serum from RA and OA patients did not differ significantly(P=0.122),but its concentration in joint fluid of RA patients was significantly higher than that of OA patients (P=0.037).In the serum,there was significant correlation between PDCD5 and TNF-α (r=-0.55,P=0.004; r=-0.51,P=0.012)in both RA and OA patients.The correlation between PDCD5 and TNF-α in joint fluid of RA patients was statistically significant(r=-0.49,P=0.012),but no correlation could be found in joint fluid between PDCD5and TNF-α of OA patients(r=-0.353,P=0.09).ConclusionThis study suggests that PDCD5 and TNF-αare important apoptosis-regulatory factors in RA,and play important roles in the occurrence and development of RA.
