Gene-expression signature of AC-cAMP signal pathway in thyroid of H22 tumor-bearing mice with different symptoms
10.3760/cma.j.issn.1673-4246.2011.11.009
- VernacularTitle:同病异证H22肝癌小鼠甲状腺AC-cAMP信号通路基因差异表达的特征
- Author:
Wenli LU
;
Zhaoqin FANG
;
Zhiqiang PAN
;
Xiaomei LIU
;
Chao LIANG
;
Dongyuan GUAN
;
Zhonghua WU
- Publication Type:Journal Article
- Keywords:
Liver cancer;
Same disease with different syndromes;
Thyroid;
Thyroid hormone;
Gene expression;
AC-cAMP signal pathway;
Mice
- From:
International Journal of Traditional Chinese Medicine
2011;33(11):988-994
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe gene transcription characteristic of AC-cAMP signal pathway in thyroid of H22 tumor-bearing mice with different syndromes.MethodsThe quantitative four diagnosis and syndrome differentiation methods and Affymetrix Gene Chip Mouse Exon 1.0 ST Array were used,thyroid gene expression of normal mice,qi-deficiency syndromes (QDS) and poisonous pathogenic factors syndrome (PPFS) in early stage of H22 tumor-bearing mice were detected.Genes of AC-cAMP signal pathway in gene chips,which related with thyroid hormone synthesis and secretion were selected,and then analyzed their expressive characteristic.Results ①In key genes,TG and Pax8 were down-regulated in early stage,TSHr down-regulated in PPFS while contrary in QDS.②the transcription level of most genes in QDS were slightly higher than in PPFS,Nis,Tpo,AC,Ttf1,Titf2 and Prkaca were included.()Slight changes showed in other genes in this pathway.ConclusionIn AC-cAMP pathway of H22 tumor-bearing mice with different symptoms,some key genes showed similar characteristics including TSHr,AC,Pax8,Ttf1,Tiff2,TG,Nis and Tpo.This suggested that the thyroid is inhibited in mice with PPFS.
