Research progress in feeder layers of human embryonic stem cells
10.3760/cma.j.issn.1673-4181.2011.05.010
- VernacularTitle:人胚胎干细胞饲养层培养体系的研究进展
- Author:
Xue WANG
;
Xiaorong MA
;
Junmei ZHOU
;
Fang CHEN
- Publication Type:Journal Article
- Keywords:
Human embryonic stem cell;
Culture system;
Feeder layer
- From:
International Journal of Biomedical Engineering
2011;34(5):298-301
- CountryChina
- Language:Chinese
-
Abstract:
Human embryonic stem cell (hES cells) lines can be derived from the inner cell mass (ICM) of preimplantation blastocysts.hES cells are commonly defined as undifferentiated pluripotent cells that can proliferate and have the capacity of both self-renewal and differentiation into one or more types of specialized cells.hES cells remain undifferentiated when culture on feeder layers,such as murine embryonic fibroblasts (MEFs).It is believed that various factors secreted from feeder layers are necessary to prevent hES cell from differentiation.In this review,we will summarize the advantages and disadvantages of various types of feeder cells by which a reference for future research will be provided.
