Study on the expression of serum amyloid protein A in vivo in patients with rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2011.11.014
- VernacularTitle:血清淀粉样蛋白A在类风湿关节炎患者体内表达的研究
- Author:
Yun MU
;
Wei WEI
;
Fujiang ZHANG
;
Xuguo SUN
;
Hongyi LIU
;
Min NI
;
Fang ZHENG
- Publication Type:Journal Article
- Keywords:
Serum amyloid A protein;
Arthritis,rheumatoid;
Immunohistochemistry
- From:
Chinese Journal of Rheumatology
2011;15(11):783-785
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the role of SAA in rheumatoid arthritis(RA) pathogenesis by analyzing the expression of serum amyloid protein A(SAA) in serum,synovial fluid and synovial membrane in patients with RA.MethodsSAA levels in the serum and synovial fluid in each group were detected.Sera SAA was tested by Western blotting,while the expression of SAA in RA and osteoarthritis(OA) synovium was detected by immunohistochemistry.Comparisons between groups were performed by t-tests or KruskaWallis test.ResultsThe serum levels of SAA were significantly higher in RA [(318±132) μg/L] than those in OA [(127±47) μg/L] and healey controls [(127±41) μg/L,P＜0.01].In RA,the SAA levels in the synovial fluid [ (571±473) μg/L ] were significantly higher when compared to those in O A [ (129±33) μg/L](t=2.46,P=0.04).Western blotting results showed that SAA bands were found in the serum samples of each group,and higher expression of SAA were seen in RA.Pathology study had showed that SAA was observed mainly in endothelial cells,synovial fibroblasts,macrophages and perivascular areas in RA synovium.In OA,SAA was observed in perivascular areas and synovial fibroblasts.ConclusionIn RA,SAA levels in both serum and synovial fluid are significantly higher than those in the controls.High expression of SAA in RA synovium can be observed.Our results suggest that SAA may play a role in inflammation reaction and joint destruction of RA.
