Effects of β-elemene combined with chemotherapy on the protein expressions in lung adenocarcinoma A549 cell line
10.3760/cma.j.issn.0254-9026.2011.11.021
- VernacularTitle:β-榄香烯联合热化疗对肺腺癌患者A549细胞系中蛋白表达的影响
- Author:
Xinhai ZHU
;
Yongqing WANG
;
Zhiming JIANG
;
Zhibing WU
- Publication Type:Journal Article
- Keywords:
Carcinoma,non-small-cell lung;
Cisplatin;
Suppressor of cytokine signaling proteins
- From:
Chinese Journal of Geriatrics
2011;30(11):962-964
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the possible molecular mechanisms of β-elemene combined with cisplatin and heat therapy for killing A549 cell line.MethodsThe protein expressions of Stat3,p21 Waf1/Cip1 and Survivin were detected with Western blot after treatment with β-elemene of different concentrations combined cisplatin and heat therapy.ResultsThe protein expressions of Stat3,and pStat3 and p21Waf1/Cip1 in A549 cell line were enhanced with increasing concentrations,and there was significant difference in the expressions between high and low concentration,but Survivin protein had no change at 37°C.After adding 4 μg/mL cisplatin,the expressions of p21Waf1/ Cip1,Survivin,Stats and pStat3 were reduced at β-elemene of high concentration.At 42°C,there was no significant difference in expression of Stat3 protein at 60 μg/mL elemene,but the expressions of pStat3,p21Waf1/Cip1 and Survivin proteins had sharply declined.When using 15 μg/mL elemene combined with 4 μg/mL cisplatin,the protein expressions of Star3 and pStat3 increased,and Survivin expression decreased.ConclusionsAt temperature of 37°C,β-elemene of high concentration may inhibit growth of A549 cells by higher expression of p21Waf1/Cip1 protein,and mainly by inhibiting expressions of Stat3 and pStat3 and Survivin after combined with cisplatin.At temperature of 42°C,β- elemene of highconcentrationmaypromoteapoptosispossiblythroughinhibition ofStat3 phosphorylation and expression of Survivin protein.β-elemene of low concentration combined with cisplatin leads to synergy killing effect by reducing expression of Survivin protein.
