Effect of radiation on collagen type I mRNA expressions in mouse osteoblast in vitro
10.3760/cma.j.issn.1673-4181.2011.05.002
- VernacularTitle:辐射对体外培养的小鼠成骨细胞Ⅰ型胶原mRNA表达的影响
- Author:
Leilei ZHONG
;
Bing YANG
;
Shuanglin WANG
;
Tiqiang FAN
;
Ying HAN
;
Fujun YANG
;
Yuanming SUN
- Publication Type:Journal Article
- Keywords:
Collagen type I;
Radiation damage;
Osteoblast
- From:
International Journal of Biomedical Engineering
2011;34(5):261-264
- CountryChina
- Language:Chinese
-
Abstract:
Objective Cancer radio-therapy may induce bone damage of the patients.collagen type I gene expressions in osteoblast after radiation indicates the influence of radiation on the function of early and late osteoblast.Methods Bone marrow stromal cells were differentiated into osteoblasts in vitro.and the characteristics was indentified.The collagen type I expressions in early and late stage osteoblasts exposed to 1～4Gy radiation were examined by RT-PCR.Results Compared to control group,collagen type I gene expressions increased in early osteoblast after 1～3 Gy radiation (P＜0.05),while the gene expressions in late osteoblast that cultured 10 days decreased.Collagen type I gene expression in late stage ostoblast after 4 Gy irradiation was greatly higher than that in early stage osteoblast (P＜0.01).Conclusion After 1～3 Gy irradiation,the collagen type I expression in early osteoblast was enhanced,indicating the increased ability of bone formation.The exposure to 1～3 Gy decreased collagen type I expression in late osteoblast and weakened the ability of bone formation.The result of high expression of collagen type I in late osteoblast after 4 Gy irradiation may be the manifestation of compensatory function.
