Sensitivity to drugs of chemotherapy of cancer stem cells of human pancreatic carcinoma
10.3760/cma.j.issn.1007-8118.2011.11.015
- VernacularTitle:胰腺癌肿瘤干细胞对抗肿瘤药物敏感性的实验研究
- Author:
Yuhe WANG
;
Fei LI
;
Shuang LIU
;
Haichen SUN
;
Yeqing CUI
- Publication Type:Journal Article
- Keywords:
Pancreatic carcinoma;
Stem cell;
Drug resistance
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(11):919-923
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo explore the sensitivity of cancer stem cells to chemotherapy in human pancreatic carcinoma.MethodsPANC-1 ceils were cultured in an incubator filled with 5％ CO2 at a temperature of 37℃,and were labeled with Hoechst 33342.The SP analysis and sorting were performed using a FACSVantage SE.RT-PCR was performed to detect the expression of human CD133 ABCG2 and Notch1.SP and non-SP cells from the PANC-1 cell line were treated with 5-fluorouracil (5-FU; 1,10,or 100 μg/ml) or gemcitabine (10,100,or 1000μg/ml),and the cell viability was determined using the MTT assay.The sensitivity of sorted tumor cells to chemotherapeutics was determined in NOD/SCID mice model.ResultsSP cells were found to have higher drug-resistance both in vivo and in vitro and higher levels of mRNA expression for CD133,ABCG2 and Notch1,when compared to non-SP ceils.The xenografted tumors derived from injected SP cells and treated with gemcitabine had more CD133± cells than the untreated group.ConclusionsThe SP of PANC-1 pancreatic carcinoma cells are enriched with highly gemcitabine-resistant CSCs and determine the carcinogenesis of the PANC-1 pancreatic carcinoma cells.
