Expression and biological function of miR-622 in gastric cancer cells
10.3760/cma.j.issn.1007-631X.2011.11.015
- VernacularTitle:miR-622在胃癌细胞中的表达及其功能的研究
- Author:
Ruizheng MIAO
;
Leping LI
;
Jianfeng LI
;
Meng WANG
;
Hongguang SHENG
;
Tao XU
- Publication Type:Journal Article
- Keywords:
Stomach neoplams;
MicmRNAs;
Gene expression
- From:
Chinese Journal of General Surgery
2011;26(11):932-935
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological function of miR-622 in human gastric cancer cell lines of SGC-7901 and NCI-N87 cells and its role in gastric carcinogenesis.Methods We analyzed the expression of miR-622 in those human gastric cancer cell lines by quantitative real-time polymerase chain reaction.Tumorigenesis,migration and invasion ability of miR-622 overexpression was assessed in vitro with miR-622 precursor and inhibitor in in SGC-7901 and NCI-N87 cells.Results The expression level of miR-622 in SGC 7901 was 1.29 ± 0.57,and it was 10.96 ± 1.02 in NCI-N87 cells.The soft agar colony formation rate was 76% in SGC-7901 after transfecting miR-331-3p precursor,the ability of scratch healing was ( 11 ±7) μm,and the ability of transwell invasion was (731 ±3),compared with that in control group,the differences were statistically significant ( P < 0.05 ).Conclusions Over-expression of miR-622 promotes tumorigenesis,migration,and invasion in gastric cancer cells in vitro.