The protective effect of glucagon-like peptide-2(GLP-2) on intestinal barrier function in bile duct ligated rats
10.3760/cma.j.issn.1007-8118.2011.11.017
- VernacularTitle:胰高血糖素样肽-2对实验性梗阻性黄疸大鼠肠道屏障功能的保护作用
- Author:
Penglei GE
;
Qianbin JIA
;
Ning LI
- Publication Type:Journal Article
- Keywords:
Glucagon-like peptide-2;
Obstructive jaundice;
Barrier function
- From:
Chinese Journal of Hepatobiliary Surgery
2011;17(11):928-931
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate the effect of glucagon-like peptide-2(GLP-2) on intestinal barrier function in the bile duct ligated rats.MethodsSeventy-two SD rats were randomly divided into three groups:GLP-2 treated group(T group),obstructive jaundice control group (C group) and sham operation group (SO group).The mRNA expression of GLP-2R was measured by semi-quantified reverse transcription polymerase chain reaction (RT-PCR)and the bcl-2 expression in the intestinal mucosa was measured by immunohistochemistry staining equipped image analyzing systems (Image proplus Version 4.5).ResultsThe mRNA expression of GLP-2 in intestinal mucosa in T group was higher than that in C group (P＜0.05) but lower than that in SO group (P＞0.05).The expression of bcl-2 in the intestinal villi of rats in C group showed more significant decrease (P＜0.05) than those in the SO and T groups especially on day 3 and 7 after operation (P＜0.05).ConclusionsGLP-2 may increase the mRNA expression of GLP-2R,stimulate the growth of intestinal mucosa,diminish the number of the apoptosis cells,and protect the intestinal barrier function in obstructive jaundice rats.
