Open field test as a method for ethology in Kunming mice: three behavioral dimensions by factor analysis
10.3760/cma.j.issn.1674-6554.2011.10.004
- VernacularTitle:昆明小鼠旷场实验评价方法的三维结构
- Author:
Shiguang SUN
;
Zifa LI
;
Peng SUN
;
Sheng WEI
;
Mingqi QIAO
;
Huiyun ZHANG
- Publication Type:Journal Article
- Keywords:
Open field test;
Kunming mice;
State anxiety;
Animal model;
Structural dimensionFactor analysis;
Correlation
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2011;20(10):875-877
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the behavioral dimensions of the open field test ( OFF),as a method for ethology in Kunming mice.Methods The behavior of adult,male,Kunming mice in OFF was recorded twice,for five minutes,with a one-week inter-trial interval.The following parameters were evaluated by factor analysis:percentage of time exploring in the central area (Ctime％); percentage of squares crossing in the central area (Ccross％) ; total number of squares crossing in the whole apparatus(Cross) ; total number of rears in the whole apparatus(Rear) ; and number of fecal boli(FB).Results There were good intercorrelation and intracorrelation between test and retest for Ctime％,Ccross％,Cross and Rear.Ctime％ and Ccross％ had significant loadings on “Anxiety” factor ( Loading％:43.34％,48.56％ ) ; Cross and Rear loaded on “locomotor-Exploration” factor ( Loading％:27.94％,29.30％ ) ; and FB loaded on “Emotionality” factor ( Loading％:21.95％,15.93％ ).A good correlation was found between test and retest for anxiety factor and locomtor-exploration factor.Conclusion Open field test,as a method for ethology in Kunming mice,comprise three behavioral dimensions:anxiety factor,OFF-activity factor(locomotor-exploration) and emotionality factor.
