Influence of CTLA4Ig and CD40Ig gene local co-transfection on the survival of xenogenic renal allografts
10.3760/cma.j.issn.0254-1785.2011.11.010
- VernacularTitle:CTLA4Ig基因和CD40Ig基因转染供肾对异种大鼠移植肾存活的影响
- Author:
Manbo CAI
;
Jianjun LI
;
Yanping WU
;
Zhigang LUO
- Publication Type:Journal Article
- Keywords:
Rats;
Kidney transplantation;
Antigens,CD40;
T-Lymphocytes,cytotoxic;
Fusion proteins
- From:
Chinese Journal of Organ Transplantation
2011;32(11):679-682
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the efficacy of CTLA4Ig and CD40Ig gene local cotransfection on the survival of renal allografts.Methods The kidneys of guinea pig were transfected with PcDNA 3.1+-CTLA4Ig and/or PcDNA 3.1+ -CD40Ig gene by Lipo2000,and the transfected kidneys were transplanted to SD rats.The recipients were divided into group 1 (transfected with PcDNA 3.1 +,mock group),group 2 (transfected with PcDNA 3.1 + -CD40Ig,CD40Ig group),group 3 (transfected with PcDNA3.1+ -CTLA4Ig,CTLA4Ig group) and group 4 (co-transfected with PcDNA 3.1+-CD40Ig and CTLA4Ig,CTLA4Ig+ CD40Ig group).The effects of CTLA4Ig and CD40Ig transfection were determined by Western blotting.The serum creatinine (Scr) in the recipients,the pathological changes of the allografts and the survival of renal allografts were observed.Results Significantly prolonged allografts survival time was observed in group 2 (40.7 ± 10.9 days),group 3 (49.3 ± 9.5 days) and group 4 (75.7 ± 8.0 days) as compared with group 1 (6.8 ± 1.9days),especially allografts got the longest survival time in group 4 (75.7± 8.0 days).The serum creatinine level was reduced in group 4 at 30th day as compared with that in group 2 and group 3.The lymphocytes infiltrating rate in the grafts was lowest in group 4 after transplantation.Conclusion Local co-transfection of CTLA4Ig and CD40Ig genes can prolong the survival time of renal allografts significantly.
