Expression of CD40L in CD4 + T cells from patients with systemic sclerosis
10.3760/cma.j.issn.1008-1372.2011.10.001
- VernacularTitle:系统性硬皮病患者外周血CD4+T细胞中CD40L表达水平的研究
- Author:
Xiaori LIAN
;
Xinhong HU
;
Rong XIAO
;
Takuro KANEKURA
;
Hongyan JIANG
;
Yan YANG
;
Yaoyao WANG
;
Yaping LI
;
Guiying ZHANG
;
Ming ZHAO
;
Qianjin LU
- Publication Type:Journal Article
- Keywords:
Scleroderma,systemic/ME;
CD40 ligand/ME;
CD4-positive T-lymphocytes
- From:
Journal of Chinese Physician
2011;13(10):1297-1300
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression levels of CD40L in CD4 + T cells from systemic sclerosis (SSc) patients.Methods PBMC ( peripheral blood mononuclear cells) cells were isolated from the peripheral venous blood of SSc patients (16females,10males) and healthy donors (15females,10males) by density gradient centrifugation.CD4 + T cells were isolated using magnetic beads.mRNA levels of CD40L in CD4 + T cells were measured by real-time quantitative polymerase chain reaction (RTPCR).Flow cytometric analysis was used to detect the CD40L protein on the surface of CD4 + T cells.Results Both CD40L mRNA and protein expression in CD4 +T cells was significantly elevated in female SSc patients compared with female healthy controls [5.61 ± 1.86 vs 2.80 ±0.94,P ＜0.01 ; (6.70 ±3.55)％vs (2.37 ± 1.39)％,P ＜ 0.05,respectively].No significant increase in CD40L mRNA and protein expression was observed in male SSc patients compared with male controls [2.59 ± 0.89 vs 1.92 ± 0.56,P ＞0.05; (2.06±1.09)％ vs (2.13±0.87)％,P ＞0.05,respectively].Conclusions CD40L was overexpressed in CD4 + T cell of female patients but not males,which maybe one of the important reasons for female susceptibility to SSc.
