Physical characteristics and tumors model contrast imaging of a self-made nano-scale microbubble contrast agent
10.3760/cma.j.issn.1004-4477.2011.10.022
- VernacularTitle:自制纳米级超声微泡体外基本特性和肿瘤造影增强显影的实验研究
- Author:
Miao ZHANG
;
Yanli GUO
;
Kaibin TAN
;
Rui LI
;
Xing HUA
;
Jun DING
- Publication Type:Journal Article
- Keywords:
Nanotechnology;
Microbubbles;
Ultrasonography;
Tumor
- From:
Chinese Journal of Ultrasonography
2011;20(10):894-897
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo investigate characteristics in vitro and contrast-enhanced effect in vivo with self-made nano-scale ultrasoud-enhanced contrast agent.MethodsNano-lipid microbubbles were prepared by machine vibration and low-speed centrifugation.The surface potential and the microbubbles size were assessed by Zetasizer nano ZS90.The bubbles dispersity and micromorphous were observed under light microscopy and electron microscope.The characteristics of microbubbles were studied at various times at 4℃ environment.The enhancement duration time and intensity of nano-lipid microbubbles in nude mice livers,kidneys and subcutaneous implanted tumors were observed,and compared with micro-scale microbubbles.ResnltsThe mean diameter of nano-lipid microbubbles was (580.6 ± 36.3) nm.The contrast imaging study in vivo showed the nano-lipid microbubbles could significantly enhance ultrasonic imaging in nude mice livers,kidneys and subcutaneous implanted tumors.ConclusionsSelf-made nano-scale ultrasoudenhanced contrast agent showed good stability and contrast-enhancement effect in vitro,and provide a experimental basis for ultrasound imaging and therapeutics of malignant tumors.
