Effects of endothelial progenitor cell combined with allogeneic bone marrow transplantation on hematopoietic reconstitution in mice
10.3760/cma.j.issn.0254-1785.2011.11.012
- VernacularTitle:HSCT联合不同剂量内皮祖细胞输注对小鼠造血重建的影响
- Author:
Guoliang SONG
;
Bin PAN
;
Kunming QI
;
Licai AN
;
Lu JIA
;
Shijuan XU
;
Shiling YAN
;
Kailin XU
;
Lingyu ZENG
- Publication Type:Journal Article
- Keywords:
Mice;
Hematopoietic stem cell transplantation;
Endothelial progenitor cells;
Hematopoietic reconstitution
- From:
Chinese Journal of Organ Transplantation
2011;32(11):688-692
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a proper dose of endothelial progenitor cells (EPCs)administration that can achieve optimal hematopoietic improving effectiveness in a murine allogeneic hernatopoietic stem cell transplantation (allo-HSCT) model.Methods Female Balb/c mice were lethally irradiated with 60Co source,and then were injected intravenously with 5 106 bone marrow cells from C57BL/6 mice (bone marrow transplantation group).In co-transfer experiments,5 × 104,1 ×105,5 × 105 or 1 × 106 donor EPCs (EPCs treated groups) were injected simultaneously with bone marrow cells.The recipients were monitored for survival,peripheral white blood cells,hematopoietic stem cells (HSCs) and bone marrow histology.Results Compared with bone marrow transplantation group,all EPCs treated groups had accelerated recovery of peripheral white blood cells (P＜0.05),platelets (P＜0.05) and HSCs (P＜0.05).When infused with less than 5 × 105 EPCs,these effective hernatopoietic improving phenomena showed a positive correlation with the administrated doses of EPCs.However,when infused with 1 × 106 EPCs,the mice showed lower survival rate (P＜0.05)and slower recovery of peripheral white blood cells (P＜0.05),platelets (P＜0.05) and HSCs (P＜0.05) than 5 × 105 EPCs treated grpup.Bone marrow histopathology analysis confirmed the above findings.Conclusion Co-transfer with donor EPCs can improve survival rate and hematopoietic reconstitution of recipient mice in allo-HSCT,and 5 × 105 EPCs should be a proper dose to achieve the best effectiveness.
