Culture and identification of microvascular endothelial cells from human endometriosis
10.3760/cma.j.issn.0529-567x.2011.10.014
- VernacularTitle:卵巢子宫内膜异位症病灶微血管内皮细胞的培养与鉴定
- Author:
Zhuo OUYANG
;
Dan LU
;
Weiyuan ZHANG
- Publication Type:Journal Article
- Keywords:
Endometriosis;
Endothelium,vascular;
Cells,cultured
- From:
Chinese Journal of Obstetrics and Gynecology
2011;46(10):773-776
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the methods of isolating and culturing human ovarian endometriosis-derived microvascular endothelial cells (OEMEC).Methods The tissues of human endometriotic cyst of ovary were finely minced with scissors,then digested by collagenase Ⅰ,Ⅱ and trypsinethylene diamine tetraacetic acid (EDTA).The cells were purified by using centrifugation of 2000 r/min speed.OEMEC were identified by light microscope and transmission electron microscope observing CD34,FⅧ-Rag and Weibel-Palade in microvascular endothelial cells.Results The OEMEC grew as confluent monolayer like cobblestones under light microscope.CD34 and FⅧ-Rag were expressed strongly,and the percentages of CD34 and FⅧ-Rag positive cells were 91.4％ and 92.5％.Weibel-Palade bodies could be observed under transmission electron microscope.The time of cell doubling proliferation was 4.5 days.Conclusion The established system of isolating OEMEC would provide lab base for studying the mechanisms of angiogenesis in endometriosis lesions.
