Polypeptide cSN50 inhibits the expression of NF-κB and its downstream factor via Importin α in HepG2 cells
10.3760/cma.j.issn.1008-6315.2011.10.015
- VernacularTitle:多肽cSN50通过核质转运受体Importinα抑制HepG2细胞核因子-KB及其下游因子的表达
- Author:
Boting CHEN
;
Hu XUE
;
Yaomin FAN
- Publication Type:Journal Article
- Keywords:
Alcoholic liver disease;
Endotoxin;
Importin α;
NF-κB;
cSN50
- From:
Clinical Medicine of China
2011;27(10):1049-1054
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether polypeptide cSN50,as a transmembrane peptides,can inhibit NF-κB nuclear translocation and its downstream gene expression to play a role to protect cells by blocking the combination of NF-κB with the importinα3 during the alcohol and endotoxin-induced inflammation and apoptosis.Methods Flow cytometry method was used to observe the apoptosis rate of HepG2 by different concentration of alcohol and/or endotoxin.Spectrophotometer method was used to detect Caspase-3 activity.TNF- α in cell culture supernatant was detected by ELISA assay.p50,importinα3,and IκBαunder the stimulation of alcohol and/or endotoxin at selected optimal concentration were detected by Western blot.Indirect immunofluorescence assay was used to measure the activation of p50,IκBα,and importinα3.Results The change of TNF-α and Caspase-3 in the dose-effect course,compared with control group,was significant (P ＜0.05 ).p50 was increased in the nucleus ( P ＜ 0.05 ),and IκBα was decreased in the cytoplasm ( P ＜ 0.05 ).cSN50( 100 μmol/L) partially blocked nuclear translocation of p50 and its downstream factor( P ＜ 0.05 ).Conclusion NF-κB nuclear translocation may play an important role in acute alcoholic liver injury.cSN50 can effectively inhibit NF-κB activity and its downstream gene expression in HepG2 cells.
