Hepatic expression of cannabinoid receptor 1 and 2 in C57 mice with experimental cirrhosis
10.3760/cma.j.issn.1008-6315.2011.10.003
- VernacularTitle:大麻素受体1和2在肝纤维化模型C57小鼠肝组织中的表达
- Author:
Lihong YE
;
Chongkui WANG
;
Yunyan LIU
;
Li YANG
;
Zhaoxia ZHAO
;
Huiqing JIANG
;
Erhei DAI
- Publication Type:Journal Article
- Keywords:
Cannabinoid receptor 1;
Cannabinoid receptor 2;
Carbon tetrachloride;
Hepatic fibrosis;
Hepatic injury;
Animal model
- From:
Clinical Medicine of China
2011;27(10):1015-1018
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate hepatic expressions and significances of cannabinoid receptor 1 (CB1) and cannabinoid receptor 2(CB2) in C57 mice with experimental cirrhosis.Methods Thirty C57 mice were randomly divided into three groups,i.e.normal control group,model control group and model colchicine group.Hepatic fibrosis model was prepared by intraperitoneal injection of carbon tetrachloride.The expressions of CB1 and CB2 in liver tissue of mice were observed by immunohistochemistry.The scores of inflammation grade (G) and fibrosis stage (S) were simultaneously performed.Results The scores of G and S in model control group and model colchicine group were significantly higher than those in normal control group( F =125.41,P =0.00; F =99.18,P =0.00).The scores of G and S in model control group were significantly higher than those in model colchicine group(P ＜0.01 ).The scores of CB1 and CB2 expressions in model control group and model colchicine group were significantly higher than those in normal control group ( F =29.27,P =0.00; F =36.99,P =0.00).The scores of CB1 and CB2 in model control group were significantly higher than those in model colchicine group( P ＜ 0.05 or P ＜ 0.01 ).There were significant relationships among scores of CB1,CB2,G and S in model control group and model colchicine group(Ps ＜0.05).As the scores of G and S became higher,the expressions of CB1 and CB2 gradually became more intensive.Conclusion The hepatic expressions of CB1 and CB2 in C57 mice with experimental cirrhosis increased significantly and have significant relationship with the grades of liver tissue inflammation and fibrosis.
