Effects ofEGbon expression of CREBandpCREBd in cortex of aging rats
10.3760/cma.j.issn.1674-6554.2011.10.008
- VernacularTitle:银杏叶片对衰老大鼠大脑皮层环腺苷酸反应元件结合蛋白表达的影响
- Author:
Huaiying WANG
;
Shaohui SHI
;
Jianhong SHI
;
Yue ZHANG
- Publication Type:Journal Article
- Keywords:
Extract of Ginkgo biloba;
cAMP response element binding protein;
Aging;
Rat;
Cortex
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2011;20(10):887-889
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effects of Ginkgo biloba extract(EGb) on expression of CREB and pCREB in cortex of aging rats.Methods Male Wistar rats were divided into three groups:young control group,old control group and EGb group.Rats in EGb group were treated with intragastric administration of EGb,while rats in the other two groups were treated with distilled water.The spatial learning and memory were evaluated by Morris water maze,and the expression of CREB,pCREB were detected by western blot.Results( 1 ) Compared with young control group (9.6 ± 2.88,41.55 ± 6.30),the swimming time and times through platform in the target quardrant of rats in old control group(6.8 ± 2.49,34.92 ± 4.56) were reduced (P ＜ 0.05 ).The times passing through the platform and the time exploring the target quadrant were more and longer in EGb group(9.4 ± 2.63,41.0 ± 6.68 ) than those in old control group(P ＜ 0.05 ).(2)Compared with rats in young control group( 1.07 ±0.33,0.26 ± 0.04),relative contents of CREB and p-CREB proteins in cortex (0.70 ± 0.21,0.13 ± 0.05 ) weredecreased in old control group(P＜0.05 ).CREB and p-CREB Levels were higher in EGb group ( 1.02 ±0.18,0.18 ±0.02)than those in old control group(P ＜ 0.05 ).Conclusion EGb can ameliorate spatial learning and memory of rats by increasing the expression of CREB and p-CREB in cortex.
