Up-regulation of monocyte chemoattractant protein-1 in 3T3-L1 adipocytes by CoCl2 induced hypoxia
10.3760/cma.j.issn.1000-6699.2011.10.018
- VernacularTitle:氯化钴诱导低氧上调3T3-L1脂肪细胞单核细胞趋化蛋白1的表达
- Author:
Zhuhua ZHANG
;
Xiaoyong LI
;
Ning LIN
;
Hongmei ZHANG
;
Shichun DU
;
Qing SU
- Publication Type:Journal Article
- Keywords:
3T3-L1 adipocytes;
CoCl2;
Hypoxia;
Hypoxia-inducible factor-1α;
Monocyte chemoattractant protein-1
- From:
Chinese Journal of Endocrinology and Metabolism
2011;27(10):853-856
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of hypoxia chemically induced by CoCl2 on the expression of monocyte chemoattractant protein-1 ( MCP-1 ) in mouse 3T3-L1 adipocytes.3T3-L1 preadipocytes were cultured in vitro and differentiated into the matured adipocytes.Cell differentiation and lipid accumulation was determined by Oil Red O staining.CoCl2 was used as a chemical hypoxia-inducible reagent to mimic hypoxic microenvironment.The effect of CoCl2 on cell viability was estimated by MTT assay.Hypoxia-inducible factor-1α(HIF-1α) expression under hypoxia was detected by realtime fluorescent PCR and Western blot,while MCP-1 expression was detected by real-time fluorescent PCR and ELISA.CoCl2 induced hypoxia led to a marked recruitment of HIF-1α in mouse 3T3-L1adipocytes.Similarly,both mRNA and protein levels of MCP-1 were up-regulated.Exposure of 3T3-L1 adipocytes to CoCl2 induced hypoxic microenvironment in vitro,and hypoxic induction of MCP-1 expression and secretion may be mediated by HIF-1 α and may contribute to chronic low grade inflammation in adipose tissue.
