The cell-mediated immune status of patients with cytomegalovirus pneumonia after renal transplantation
10.3760/cma.j.issn.0254-1785.2011.10.004
- VernacularTitle:肾移植术后并发巨细胞病毒肺炎患者的细胞免疫状态
- Author:
Heli XIANG
;
Wujun XUE
;
Puxun TIAN
;
Xiaoming DING
;
Xiaoming PAN
;
Hang YAN
;
Jun HOU
;
Xinshun FENG
- Publication Type:Journal Article
- Keywords:
Kidney transplantation;
CD4-Positive T-Lymphocytes;
Adenosine triphosphate;
Immunity,cellular;
Cytomegalovirus
- From:
Chinese Journal of Organ Transplantation
2011;32(10):592-595
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the clinical value of adenosine triphosphate (ATP) determination in CD4+ cells in cytomegalovirus pneumonia after renal transplantation.Methods The ATP level of CD4+ T cells was measured by ImmuKnowTM kit.The ATP levels were determined in 187 renal transplant recipients before and 30,60,90,180 days after operation,and at the time of CMV pneumonia and 4 weeks after treatment of CMV pneumonia.The associations between ATP levels and CMV pneumonia were analyzed.Analysis of variance (ANOVA),Pearson-Spearman and relative risks were used for data analysis.Results 17 cases out of 187 renal transplant recipients were diagnosed as CMV pneumonia (9.1％),and the onset of CMV pneumonia started on the (2.8 ±1.2)month after renal transplantation.ATP concentrations in CD4+ T cells were significantly lower after operation than those before operation (P＜0.01).ATP concentrations reached the lowest on the about postoperative day 90 (P＜0.05),then increased gradually.In 17 recipients with CMV pneumonia,the ATP levels before and 30,90 days after operation,at the time of CMV pneumonia and 4th week after treatment of CMV pneumonia were (376 ±182),(283 ± 146),(196 ± 112),(145 ± 102) and (236 ± 117) μg/L respectively.ATP levels at the time of CMV pneumonia were significantly lower than any other time points (P＜0.05).There was close correlation between ATP levels and CMV pneumonia.Conclusion The determination of ATP in CD4+ cells could reflect the status of cell-mediated immunity in renal transplant recipients,and could evaluate the severity and prognosis of CMV pneumonia and guide the clinical treatment.
