The expression and significance of ubiquitin in skeletal muscle of mouse with emphysema
10.3760/cma.j.issn.0254-9026.2011.10.018
- VernacularTitle:泛素在肺气肿小鼠骨骼肌中的表达和意义
- Author:
Jinfeng WANG
;
Yunqiao LI
;
Lijun BAI
;
Jing GE
- Publication Type:Journal Article
- Keywords:
Pulmonary emphysema;
Pulmonary disease,chronic obstructive;
Ubiquitin;
Muscle,skeletal
- From:
Chinese Journal of Geriatrics
2011;30(10):854-857
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes in mRNA and protein expression of ubiquitin and to explore the relationship of apoptosis in the skeletal muscle of mouse with emphysema.Methods Emphysema model was established by passive cigarette smoking in mouse.Apoptosis was detected with TUNEL staining.The reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemical assay were used to determine the mRNA and protein level of ubiquitin.Results Apoptotic cells were increased in skeletal muscle of mouse with emphysema.The mRNA and protein level of ubiquitin were significantly higher in mouses with emphysema (0.48±0.02 and 0.23+0.05,respectively) than in control group (0.17±0.01 and 0.14+0.01,t=6.223、4.093,both P ＜0.05).Conclusions The increase of apoptosis in skeletal muscle of mouses with emphysema may be associated with high expression of ubiquitin.
