Study on ganoderma acid in the treatment of rats essential hypertension
10.3760/cma.j.issn.0254-9026.2011.10.019
- VernacularTitle:灵芝酸治疗大鼠原发性高血压的实验研究
- Author:
Dongsheng ZHAO
;
Wenyuan ZHAO
- Publication Type:Journal Article
- Keywords:
Rats,inbred SHR;
Ganoderma lucidum;
Endothelial cells;
Gene expression
- From:
Chinese Journal of Geriatrics
2011;30(10):858-860
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of ganoderma acid in therapy of the rat essential hypertension.Methods 30 male spontaneously hypertensive rat (SHR) were divided randomly into three groups:control group (CG),ganoderma acid group (Gan) and captoril (Cap) as positive control group.SHR were fed with distilled water (10 ml/kg) in control group,ganoderma acid (0.6 g/kg ) in Gan group,catopril (0.03 g/kg) in Cap group.After 8-weeks therapy,5 ml blood was extracted,rats in every group were sacrificed to get the aorta.The mRNA expression of superoxide dismutase (SOD),nitric oxide synthase (NOS) and high density lipoprotein (HDL) were detected by RT-PCR and the apoptosis of endothelial cells were measured by flowcytometry.Results After the 8 weeks therapy,as compared with the CG group,blood pressure of SHR in Gan group became stable[(112.6±3.2) mm Hg](P＜0.01).The expression of SOD,NOS and HDL showed obviously upregulated,and apoptosis of endothelial cell decreased significantly (P＜0.01).NO contents increased,and MDA,ET-1 decreased (P＜0.01).The contents of LDL decreased and HDL increased (P＜0.01).Conclusions Ganoderma acid may improve the function of endothelial cell and decrease the blood pressure by promoting the mRNA expression and activity of SOD,cleaning the radical ions and inhibiting the apoptosis of the endothelial cell.
