In vitro expansion of human CD8+ CD28- suppressor T cells and their immunological regulatory effect with antigen specificity
10.3760/cma.j.issn.0254-1785.2011.10.010
- VernacularTitle:人CD8+CD28-抑制性T淋巴细胞的体外扩增及其抗原特异性调节作用
- Author:
Yuming YU
;
Yanjun LIU
;
Wenfeng DENG
;
Lixin YU
- Publication Type:Journal Article
- Keywords:
Monocytes;
Cell culture techniques;
CD8-positive T-lymphocytes;
T-lymphocytes,suppressor-effector
- From:
Chinese Journal of Organ Transplantation
2011;32(10):614-617
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the In vitro expansion of human CD8+ CD28- suppressor T cells and their immunological regulatory effect.Methods Human CD8 + CD28- suppressor T cells were expanded in vitro driven by the combination of cytokines and allogeneic antigen presenting cells (APCs).Flow cytometry was used to assess the development of CD28- subpopulation.Expanded CD8+ CD28- T cells were isolated by immunomagnetic microspheres and then added as third part modulators into mixed lymphocyte culture to assess their immunological regulatory characteristics.Results The combination of cytokines included IL-2,IL-7 and IL-15 and allogeneic APCs could increase the portion of CD8 + CD28- T cell subtype,and expansion fold of CD8+ CD28- T cell subtype was significantly increased as compared with others (P＜0.05).Expanded CD8+ CD28- T cells could suppress the proliferation of CD4+ T cells stimulated by allogeneic APCs.Moreover,this suppression had antigen specificity.Conclusion Human CD8 + CD28- suppressor T cells can be in vitro expanded in large amounts driven by the combination of cytokines and allogeneic APCs.Expanded CD8 + CD28- T cells in this study have antigen specific regulatory characteristics.
