"Comparison of roles of Toll-like receptor-4 in acute lung injury induced by lipopolysaccharide and by hemorrhagic shock and resuscitation and lipopolysaccharide ("" two-hit"") in mice"
10.3760/cma.j.issn.0254-1416.2011.08.020
- VernacularTitle:Toll样受体4在内毒素和“两次打击”致小鼠急性肺损伤中作用的比较
- Author:
Chang CHEN
;
Fan ZHANG
;
Zongze ZHANG
;
Mian PENG
;
Yanlin WANG
- Publication Type:Journal Article
- Keywords:
Toll-like receptor 4;
Respiratory distress syndrome,adult;
Endotoxemia;
Shock,hemorrhagic
- From:
Chinese Journal of Anesthesiology
2011;31(8):974-976
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo compare the roles of Toll-like receptor-4 (TLR-4) in the acute lung injury (ALI) induced by lipopolysaccharide (LPS) and by hemorrhagic shock and resuscitation (HSR) and LPS (twohit) in mice.MethodsTwo types of mice were used in this study:free wild type mice (C3H/HeN) and TLR4 gene mutation type ( C3H/HeJ).Each type of mice was randomly divided into 2 groups ( n ＝ 18):group sham operation+ LPS (group S/LPS) and group HSR + LPS (group HSR/LPS).Hemorrhagic shock was induced by blood withdrawal until MAP was reduced to 35-45 ram Hg and maintained for 60 min (first hit).The animals were then resuscitated by infusion of shed blood and lactated Ringer' s solution.LPS 30 tμg/kg was instilled into trachea at 24 h after HSR (second hit).Arterial blood gas analysis was performed and the animals were then sacrificed by exsanguination at 0,3 and 6 h after LPS(T,,T2,T3 ).The lungs were removed.W/D lung weight ratio and MPO activity,IL-6 and 1L-10 contents in the lung tissue were determined.The changing rate of the abovementioned variables at T2,T3 based on the values at T1 were calculated.ResultsIn C3H/HeN animals the changing rate of PaO2 was significantly lower while the changing rate of W/D ratio,MPO activity and IL-6,IL-10 contents in the lung tissue were significantly higher in HSR/LPS group than in S/LPS group at T2.3.But in C3H/HeJ animals the above-mentioned variables were changed at T2.ConclusionThe role of TLR-4 in the two-hit-induced ALI is stronger than that in the LPS-induced ALI in mice.
