JNK signal pathway in acute necrotizing pancreatitis associated lung injury in rats
10.3760/cma.j.issn.1674-1935.2011.05.010
- VernacularTitle:JNK信号通路在急性坏死性胰腺炎大鼠相关性肺损伤中的作用
- Author:
Liangliang SHI
;
Mingdong LIU
;
Hao ZHU
;
Min CHEN
;
Xiaoping ZOU
- Publication Type:Journal Article
- Keywords:
Pancreatitis,acute necrotizing;
Lung injury;
JNK mitogen-activated protein kinases
- From:
Chinese Journal of Pancreatology
2011;11(5):336-338
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of JNK signal pathway in acute necrotizing pancreatitis (ANP) associated lung injury.Methods A total of 24 SD rats were randomly divided into sham group and ANP group,ANP rats were induced by retrograde injection of 5％ sodium taurocholate into common bile duct.The rats were sacrificed 12 and 24h later,and the pancreas and lung tissue were resected and underwent routine pathologic examination,ELISA method was used to detect the level of TNF-α and IL-1β in lung tissue.Expression of JNK mRNA was detected by real time PCR,and the expression of JNK protein were evaluated by Western blotting.Results There was bleeding,necrosis,large amount of inflammatory cells infiltration in pancreatic tissue; and there was edema,pulmonary consolidation,interstitial edema,inflammatory cells infihration in lung tissue in ANP group.The levels of TNF-α and IL-1β,JNK mRNA,JNK protein,phosphorylation JNK were ( 374.3 ± 124.0) pg/ml,(649.0 ± 114.9) pg/ml,2.57 ± 0.76,1.40 ± 0.81,0.81 ± 0.20 in ANP group,which were significantly higher than those in with sham group[( 218.2 ± 68.4)pg/ml,(524.3±58.4)pg/ml,1.03±0.11,0.32±0.11,0.32±0.11,P＜0.05].Conclusions JNK signal pathway plays an important role in experimental ANP associated lung injury in rats.
